Наукова електронна бібліотека
періодичних видань НАН України
періодичних видань НАН України
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy
Репозиторій DSpace/Manakin
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Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy
Тема:
Short communications
Посилання:
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy / A.L.S. Galbiatti, H.C. Caldas, J.A. Padovani Junior, E.C. Pavarino, E.M. Goloni-Bertollo // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 367-369. — Бібліогр.: 22 назв. — англ.
Підтримка:
Fundação de Amparo а Pesquisa do Estado
de Sгo Paulo (FAPESP) for their financial support
(Nє 2010/12930-4, 2010/12932-7); Prof. Dr. Moacir F.
Godoy for their help in statistical analysis; Profa. Dra
Eloiza Helena Tajara for providing of the cell line; Oncology
Department, Hospital de Base, São José do Rio
Preto for providing of the Methotrexate Chemotherapeutic;
CNPQ (National Counsel of Technological and
Scientific Development) and FAMERP/FUNFARME.
Дата:
2012
Переглядів:
524
Завантажень:
167
Анотація:
Aim: Methotrexate (MTX) is an antifolate agent that acts inhibiting purine and pyrimidine synthesis. The objective of the study was to evaluate the viability of Hep-2 human laryngeal cancer cells to the treatment with MTX chemotherapy in vitro. Methods: Cultured Hep-2 cells were treated with 0.25, 25.0 and 75 μM MTX for 24 h, and their viability was evaluated with Bcl-2-FITC antibody in flow cytometry. Results: The numbers of viable Hep-2 cells after 24 h treatment with 0.25, 25.0 and 75.0 uM MTX were 85.43%, 22.46% and 8.42%, respectively (p < 0.05). Therefore, MTX possesses a dose-dependent effect on viability of Hep-2 cells in vitro. Conclusion: The highest MTX concentration is associated with highest tumor cell sensitivity of human laryngeal cancer cells of Hep-2 line.
