Реєстрація зразків-стандартів алелів локусів високомолекулярних субодиниць глютенінів Aegilops biuncialis Vis.

Abstract

Aims. The objective of the investigation was selection and registration of Aegilops biuncialis Vis. accessions that may serve as standards for high-molecular weight glutenin subunit alleles. Methods. Ae. biuncialis accessions derived from Crimean populations were propagated on the experimental plot. SDS electrophoresis of high-molecular weight glutenin subunits was used to identify alleles at the Glu-U1 and Glu-Mb1 loci. Results. The most frequent alleles among the collection of propagated accessions of Ae. biuncialis were Glu-U1b and Glu-Mb1a. Fifteen accessions were registered at the National Centre of Plant Genetic Resources of Ukraine. The set of registered accessions includes six different alleles at the Glu-U1 locus and seven alleles at Glu-Mb1. Conclusions. Fifteen accessions of Ae. biuncialis registered in the National catalogue of NCPGRU may serve as standards for six alleles at the Glu-U1 locus and seven alleles at Glu-Mb1.

Key words: Aegilops biuncialis Vis., high-molecular weight glutenin subunits, alleles, registration.