Перегляд за темою "Molecular and Cell Biotechnologies"

Результати 1-20 з 53

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Action of booster immunization with E2 CSFV on immune response elicited by marker DNA-vaccine against CSF

Pokholenko, Ia.O.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2012)

Цель. Исследовать влияние бустерной иммунизации рекомбинантным фрагментом Е2 ВКЧС на гуморальный иммунный ответ, индуцированный кандидатной маркированной ДНК-вакциной против КЧС. Методы. Наличие фрагмента гена Е2 ВКЧС ...
Adhesion and proliferation of adipose derived mesenchymal stromal cells on chitosan scaffolds with different degree of deacetylation

Rogulska, O.Yu.; Petrenko, Yu.O.; Kalinkevich, O.V.; Kalinkevich, A.N.; Petrenko, O.Yu. (Вiopolymers and Cell, 2014)

Aim. Selection of the optimal scaffold for the creation of tissue engineering constructs is a key challenge of biotechnology. In this study we investigated the biocompatibility of human adipose derived mesenchymal stromal ...
A dual-target strategy for the detection of Chlamydia trachomatis by real-time PCR

Vitrenko, Y.A.; Deryabin, O.M. (Вiopolymers and Cell, 2018)

Aim. Polymerase chain reaction (PCR) is a key method for the C. trachomatis diagnostics. The first-generation tests targeting a cryptic plasmid showed quite a high sensitivity; however their value has recently been compromised ...
Advancing recovery and cryopreservation of rat spermatogonia for germ stem cell banking

Syvyk, T.L.; Djachenko, L.S.; Syvyk, A.E. (Вiopolymers and Cell, 2018)

Aim. Optimisation of spermatogonial cryopreservation medium and spermatogonia recovery procedures essential for the male germ cell research and spermatogonia mediated transgenesis in rodents. Methods. PCR, LIVE/DEAD® Cell ...
A mobile genetic element in Serratia marcescens, a causative agent of onion disease

Ovcharenko, L.P.; Voznyuk, T.M.; Zaetz, I.E.; Potopalsky, A.I.; Reva, O.N.; Kozyrovska, N.O. (Вiopolymers and Cell, 2010)

Aim. To screen mobile genetic elements (MGE) in the bacterium which caused decay of field-grown onion bulb and to study an integron and gene cassettes associated. Methods. Polymerase chain reaction (PCR) and PCR products ...
Amperometric glucose biosensor with the IrNPs/Ludox – modified enzyme matrix

Shkotova, L.V.; Woloshina, I.M.; Kovalchuk, V.V.; Zhybak, M.T.; Dzyadevych, S.V. (Вiopolymers and Cell, 2018)

Aim. To develop an amperometric biosensor based on glucose oxidase (1.1.3.4) from Aspergillus niger immobilized in the IrNPs/Ludox/GOx matrix for glucose detection. Methods. To achieve a highly selective and sensitive ...
Antioxidant capacity and sustainability of Saccharomyces cerevisiae cells exposed to ozone

Goriacha, I.P.; Zinchenko, V.D.; Dyubko, T.S.; Romodanova, E.A.; Tatarets, A.L. (Вiopolymers and Cell, 2014)

Aim. The investigation of response of S. cerevisiae cells to the effect of ozone in different doses and determination of total antioxidant capacity (TAC) of these cells. Methods. TAC was estimated with the chemiluminescent ...
Application of silicalite for improvement of enzyme adsorption on the stainless steel electrodes

Pyeshkova, V.N.; Dudchenko, O.Y.; Soldatkin, O.O.; Kucherenko, I.S.; Ozansoy Kasap, B.; Akata Kurc, B.; Dzyadevych, S.V. (Вiopolymers and Cell, 2014)

Aim. Improvement of analytical characteristics of an enzyme biosensor based on new inexpensive perspective stainless steel electrodes using silicalite nanoparticles. Methods. Conductometric enzyme biosensor was used. ...
Bioaffinity sorbent based on immobilized protein A Staphylococcus aureus: development and application

Gorbatiuk, O.B.; Tsapenko, M.V.; Pavlova, M.V.; Okunev, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2012)

Цель. Создание биоаффинного сорбента на основе ориентированно иммобилизованного белка А Staphylococcus aureus (SPA) через два целлюлозосвязывающих домена (СBD) Clostridium thermocellum и его применение для очистки антител. ...
Biological properties of neural crest-derived multipotent stem cells from the bulge region of whisker follicle expanded in new culture conditions

Vasyliev, R.G.; Rodnichenko, A.E.; Zubov, D.A.; Rymar, S.Y.; Gubar, O.S.; Labunets, I.F.; Novikova, S.N. (Вiopolymers and Cell, 2014)

Aim. The work is aimed at obtaining the culture of neural crest-derived multipotent stem cells (NC-MSCs) in new culture conditions and to investigate their biological properties. Methods. NC-MSCs were grown from the ...
Characterization of novel peptide-specific antibodies against the translation elongation factor eEF1A2 and their application for cancer research

Shalak, V.F.; Vislovukh, A.A.; Novosylna, O.V.; Khoruzhenko, A.I.; Kovalenko, M.I.; Kolesanova, E.F.; Egorova, E.A.; Mishin, A.A.; Krotevych, M.S.; Skoroda, L.V.; Negrutskii, B.S. (Вiopolymers and Cell, 2014)

Aim. We intend to characterize the new peptide-specific antibodies against the isoform 2 of translation elongation factor 1A (eEF1A2) and determine its presence in the postoperative samples of human breast, lung and stomach ...
Characterization of vaccine and field IBDV strains in Ukraine for proper vaccine selection for disease prevention

Pastyria, A.S.; Budzanivska, I.G.; Polischuk, V.P. (Вiopolymers and Cell, 2018)

Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chickens and is distributed worldwide. Primary viral antigen is VP2. The VP2 gene contains hypervariable re-gion (VP2 HRV). Mutations in ...
Colorimetric biomimetic sensor systems based on molecularly imprinted polymer membranes for highly-selective detection of phenol in environmental samples

Sergeyeva, T.A.; Chelyadina, D.S.; Gorbach, L.A.; Brovko, O.O.; Piletska, E.V.; Piletsky, S.A.; Sergeeva, L.M.; El’skaya, A.V. (Вiopolymers and Cell, 2014)

Aim. Development of an easy-to-use colorimetric sensor system for fast and accurate detection of phenol in envi- ronmental samples. Methods. Technique of molecular imprinting, method of in situ polymerization of molecularly ...
Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells

Sukach, A.N.; Shevchenko, M.V.; Liashenko, T.D. (Вiopolymers and Cell, 2014)

Aim. To study the influence of fetal bovine serum and serum of adult rats on behavior of newborn rat isolated neural cells during their cultivation in vitro. Methods. The isolation of neural cells from neonatal rat brain. ...
Construction and analysis of the transgenic carrot and celery plants expressing the recombinant thaumatin II protein

Luchakivska, Yu.S.; Komarnytskii, I.K.; Kurchenko, I.M.; Yurieva, O.M.; Zhytkevich, N.V.; Kuchuk, M.V. (Вiopolymers and Cell, 2015)

Aim To obtain the transgenic carrot and celery plants able to express recombinant thaumatin II in order to increase plant stress tolerance. Methods. Agrobacterium-mediated transformation of the carrot and celery seedlings ...
Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut

Gorbatiuk, O.B.; Okunev, O.V.; Nikolaev, Yu.S.; Svyatenko, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2013)

Aim. The creation of genetically engineered fusion protein SPA-BAPmut and its application as a secondary immunoreagent in immunoassays. Methods. Gene cloning, PCR, electrophoresis, DNA sequencing, bacteria cells culturing, ...
Crambe aspera plants in vitro propagation and its effect on fatty acids and phenolic compounds content and genome stability

Pushkarova, N.O.; Lakhneko, O.R.; Morgun, B.V.; Kuchuk, M.V.; Blume, Ya.B.; Yemets, A.I. (Вiopolymers and Cell, 2019)

In vitro culture can be used for endangered species preservation but its effect on biochemical properties and genetic stability of plants requires further research. Aim. The study on fatty acids content, phenolic compounds ...
Creation of glyphosate-resistant Brassica napus L. plants expressing DesC desaturase of cyanobacterium Synechococcus vulcanus

Sakhno, L.O.; Gerasymenko, I.M.; Komarnitsskii, I.K.; Sheludko, Y.V.; Goldenkova-Pavlova, I.V. (Вiopolymers and Cell, 2012)

Aim. Creation of glyphosate-resistant canola plants expressing bifunctional hybrid desC::licBM3 gene. In the hybrid gene the sequence of DesC desaturase of cyanobacterium S. vulcanus without plastid targeting was fused with ...
Detection of stress resistance genes in transgenic maize by multiplex and touchdown polymerase chain reaction

Bannikova, M.A. (Вiopolymers and Cell, 2015)

Aim. To develop a methodology for detection of the genes of resistance to the stress factors in transgenic maize by multiplex (mPCR) and touchdown polymerase chain reactions. Methods. isolation of total DNA by CTAB method, ...
Development and characterization of porous functionalized collagen scaffolds for delivery of FGF-2

Pokholenko, Ia.O.; Chetyrkina, M.D.; Dubey, L.V.; Dubey, I.Ya.; Moshynets, O.V.; Sheludko, E.V.; Shpylova, S.P.; Degtiarova, M.I.; Kordium, V.A. (Вiopolymers and Cell, 2014)

Aim. To develop the porous functionalized collagen scaffold for the delivery of FGF-2 and studying its properties in vitro and in vivo. Methods. Porous collagen scaffolds were prepared by freeze- drying collagen I solutions ...

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