Альфа-Е-катенін у гістологічних перебудовах міокарда при старінні

Abstract

Aim. In our current work we have focused on cardiospecific ablation of alpha-E-catenin during cardiogenesis and its reflection on postnatal heart. Methods. Kaplan-Meier analysis of mutant and WT mice survival. Histological analysis (hematoxylin-eosin and van Gieson stain) of old transgenic and wild-type animals. The HW/BW and HW/TL index was calculated to estimate myocardium hypertrophy development. Results. Alpha-E-catenin deletion leads to shorten life span of mutant mice. We observed increased heart weight, histological abnormalities of myocardium and increased fibrosis in hearts of mice with alpha-E-catenin deletion. Conclusions. We have shown that embryonic cardiac specific deletion of such as one as well as both alleles of the alpha-E-catenin gene leads to disorders of the heart structure, which typical of dilated cardiomyopathy, ischemic heart disease, accompanied by fibrosis. As a result all this violation of heart tissue structure occur early death of animals. We assume that the loss of alpha-E-catenin leads to such dramatic consequences not only due to violation of cells interactions, but deregulation of signalling passways in cardiomyocytes, it’s should be clarified with further molecular genetics and physiological studies. Keywords: α-catenin, gene deletion, heart, heart development.