Застосування наноматеріалу в ембріогенетичній системі in vitro отримання ембріонів свиней

Abstract

Aims. The optimization model culturing somatic and germ cells of animals through the use of nanomaterials currently is an important task in the system of development and improvement of effective ways to get in vitro oocytes preimplantant embryos and embryonic stem cells. Methods. To research the oocyte-cumulus complexes (OCC), pigs were divided into two groups: the experimental group, in which cultivation was carried out in a medium containing 0.001 % concentration of UFS / N-Gal and the control group, in which cultivation OCC conducted without the addition of nanomaterials. Ejaculated cryopreserved sperm of boar was used for in vitro fertilization of pig’s oocytes. Results. Morphological and cytogenetic analysis showed that the level of maturation of oocytes in vitro in the experimental group was 13.6 % higher compared to the control group (70.6 % ± 3.6). To investigate the in vitro maturation of oocytes pigs spent their fertilization of cryopreserved boar sperm. The quantity of zygotes in the experimental group was formed outside the body by 11.0 % more than in the control group (31.8 % ± 4.9), also level splitting of embryos was higher at 15.1 % in the experimental group compared with controls (18.2 % ± 4.1). Conclusion. The system of optimization in conditions of in vitro biological activity of nanomaterials was developed and the methods of biotechnological manipulation of gametes females in the system of rational use and reproduction of native breeds of farm animals were improved. Keywords: oocytes, in vitro fertilization, embryos, nanomaterial, N-galactosamine, cytogenetic analysis.