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Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells

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dc.contributor.author Poh, Y.W.
dc.contributor.author Gan, S.Y.
dc.contributor.author Tan, E.L.
dc.date.accessioned 2018-06-19T11:45:46Z
dc.date.available 2018-06-19T11:45:46Z
dc.date.issued 2012
dc.identifier.citation Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells / Y.W. Poh, S.Y. Gan, E.L. Tan // Experimental Oncology. — 2012. — Т. 34, № 2. — С. 85-89. — Бібліогр.: 40 назв. — англ. uk_UA
dc.identifier.issn 1812-9269
dc.identifier.uri http://dspace.nbuv.gov.ua/handle/123456789/138694
dc.description.abstract The aim of this study is to investigate whether IL-6, IL-10 and TGF-β are able to confer resistance to apoptosis in nasopharyngeal carcinoma cells by upregulating the expression of survivin. Methods: The human nasopharyngeal carcinoma cell line TW01 (WHO NPC Type I) was cultured in DMEM-F12 Ham medium containing 10% FBS in a humidified atmosphere of 5% CO2 and 37◦C and treated with different concentrations of IL-6, IL-10 and TGF-β. Survivin mRNA expression was measured by real-time quantitative PCR and Western blot. Apoptosis was determined based on the assay for caspase-3 activity. Results: Of all the cytokines tested, only TGF-β (10 pg/mL) induced the over-expression of survivin at a significant level and this correlated with resistance to apoptosis (p ≤ 0.05). To confirm if survivin is responsible for resistance to apoptosis, YM155 which is a survivin inhibitor was used and the results showed that YM155 abrogated the protective effect of TGF-β. Interestingly, IL-10 did not significantly alter the expression of survivin. Conclusions: We conclude that TGF-β up-regulates the expression of survivin leading to the resistance to apoptosis in NPC TW01 cells. uk_UA
dc.description.sponsorship We thank the staff of IMU Research Laboratories for their continuous support and enthusiasm. The study was financed by the International Medical University (IMU). uk_UA
dc.language.iso en uk_UA
dc.publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України uk_UA
dc.relation.ispartof Experimental Oncology
dc.subject Original contributions uk_UA
dc.title Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells uk_UA
dc.type Article uk_UA
dc.status published earlier uk_UA


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