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Characteristics of homocysteine-induced multidrug resistance of human MCF-7 breast cancer cells and human A2780 ovarian cancer cells

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dc.contributor.author Lukyanova, N.Yu.
dc.date.accessioned 2018-06-19T10:10:27Z
dc.date.available 2018-06-19T10:10:27Z
dc.date.issued 2010
dc.identifier.citation Characteristics of homocysteine-induced multidrug resistance of human MCF-7 breast cancer cells and human A2780 ovarian cancer cells / N.Yu. Lukyanova // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 10-14. — Бібліогр.: 38 назв. — англ. uk_UA
dc.identifier.issn 1812-9269
dc.identifier.uri http://dspace.nbuv.gov.ua/handle/123456789/138589
dc.description.abstract Aim:To study the influence of homocysteine on the mechanisms of drug resistance formation. Methods: In current study human MCF-7 breast cancer cells and A2780 ovarian cancer cells sensitive to anticancer drugs were used. To access the viability of cells, we applied 3-[4,5-dimethylthiazol-2–1]-2,5-diphenyltetrazolium bromide colorimetric assay (MTT-test). Expression of Bcl-2, p-glycoprotein (P-gp), glutathione S-transferase (GST) and E-cadherin was studied by immunocytochemistry. Results: A2780 and MCF-7 cells were treated by homocysteine. It was shown that every next treatment with homocysteine (up to 5th) decreased the sensitivity of A2780 and MCF-7 cells to cytotoxic drugs. Immunocytochemical study of molecular profile of A2780 and MCF-7 cells after long-term cultivation with homocysteine has been carried out and has revealed that such treatment resulted in the induction of Bcl-2, P-gp, GST and E-cadherin expression. This indicates that incubation of studied cells with homocysteine leads to simultaneous induction of expression of drug resistance markers to cisplatin and doxorubicin. Conclusion: Cultivation of MCF-7 and A2780 cells with homocysteine leads to simultaneous development of resistance to doxorubicine and cisplatin. The development of drug resistance is diverse for different drugs and varies among cell lines. uk_UA
dc.description.sponsorship Author gratefully acknowledges Head of Department of Mechanisms of Anticancer Therapy of R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, academician V.F. Chekhun and collaborators for valuable advices and comments. uk_UA
dc.language.iso en uk_UA
dc.publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України uk_UA
dc.relation.ispartof Experimental Oncology
dc.subject Original contributions uk_UA
dc.title Characteristics of homocysteine-induced multidrug resistance of human MCF-7 breast cancer cells and human A2780 ovarian cancer cells uk_UA
dc.type Article uk_UA
dc.status published earlier uk_UA


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