Наукова електронна бібліотека
періодичних видань НАН України
періодичних видань НАН України
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Репозиторій DSpace/Manakin
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Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Тема:
Original contributions
Посилання:
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ.
Підтримка:
All authors claim no conflicts of interest or financial involvements with this manuscript. This study was funded by Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and by Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital.
Дата:
2017
Переглядів:
1138
Завантажень:
458
Анотація:
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.
