Лечебное действие антидисбиотического препарата «Квертулидон» на состояние тканей полости рта крыс при антихеликобактерной терапии

Abstract

Антихеликобактерная терапия (АХБТ), которая включала омепразол, амоксил и кларитромицин, вызывает развитие в ротовой полости дисбиоза и воспаления, увеличение атрофии альвеолярного отростка и числа кариозных поражений. Аппликации орального геля «Квертулидон» (кверцетин + инулин + имудон + цитрат кальция) устраняет патологические явления в ротовой полости, возникающие при АХБТ.

Антихелікобактерна дія (АХБТ), яка складалась з омепразолу, амоксилу і кларітроміцину, викликає розвиток в ротовій порожнині дисбіозу, запалення, збільшення атрофії альвеолярного відростка та числа каріозних уражень. Аплікації геля «Квертулідон» (кверцетин + інулін + імудон + цитрат кальція) усувають патологічні явища в ротовій порожнині за умов АХБТ.

Aim: To determine the therapeutic effect of the oral gel “Qertulidon” on the state of oral tissues of rats exposed to anti-helicobacter pylori therapy (AHBT). Materials and methods: Rats have received AHBT of the following composition for 8 days: omeprazole (1.3 mg/kg/day), amoxil (50 mg/kg/day) and clarithromycin (7.5 mg/kg/day). A part of AHBT treated rats have received oral applications of “Qertulidon” gel (quercetin + inulin + imudon + calcium citrate) produced by “Biochimtech” (Odessa) according to TI U 20.4-13903778-032:2012 from the first day on for 11 days. After rats euthanasia on day 12 there were determined the levels of leukocytes and leukocyte formula in the blood. The activity of urease, lysozyme, elastase, catalase and the content of malondialdehyde (MDA) were determined in the homogenates of buccal mucosa and gums. The degree of dysbiosis by Levitsky was calculated as the ratio of the relative activities of urease and lysozyme. Prooxidant-antioxidant index (PAI) was calculated as the ratio of the catalase activity and MDA content. On a dedicated mandible of rats there was determined the degree of atrophy of the alveolar process after the Nikolaeva‘s method, and also there was found the number of carious lesions. Results: «Qertulidon” increases leukocytosis and the portion of neutrophils; it decreases the degree of dysbiosis and the levels of inflammation markers (elastase and MDA) in oral tissues; it increases PAI. “Qertulidon” reduces the degree of atrophy of the alveolar process and the number of carious lesions. Conclusion: AHBT causes the development of oral dysbiosis and inflammation, which are significantly reduced by the application of gel “Qertulidon”.