Перегляд за автором "Negrutskii, B.S."

Результати 1-15 з 15

Characterization of novel peptide-specific antibodies against the translation elongation factor eEF1A2 and their application for cancer research

Shalak, V.F.; Vislovukh, A.A.; Novosylna, O.V.; Khoruzhenko, A.I.; Kovalenko, M.I.; Kolesanova, E.F.; Egorova, E.A.; Mishin, A.A.; Krotevych, M.S.; Skoroda, L.V.; Negrutskii, B.S. (Вiopolymers and Cell, 2014)

Aim. We intend to characterize the new peptide-specific antibodies against the isoform 2 of translation elongation factor 1A (eEF1A2) and determine its presence in the postoperative samples of human breast, lung and stomach ...
Control of the amount and functionality of the eEF1A1 and eEF1A2 isoforms in mammalian cells

Negrutskii, B.S.; Novosylna, O.V.; Porubleva, L.V.; Vislovukh, A.A. (Вiopolymers and Cell, 2018)

Aim. To review mechanisms of regulation of expression and the functionality of two isoforms of translation elongation factor eEF1A in mammalian cells. Results. eEF1A1 and eEF1A2 proteins are regulated by post-translational ...
Interaction of different tRNAs with translation elongation factors 1A from lower and higher eukaryotes

Futernyk, P.V.; Negrutskii, B.S.; El'skaya, A.V. (Вiopolymers and Cell, 2009)

The work is aimed at confirmation of earlier assumed mechanism of tRNA channeling. Methods. The methods of band shift assay and Forsters resonance energy transfer were used. Results. The affinities of mammalian tRNAs for ...
Isoforms of elongation factor eEF1A may be differently regulated at post-transcriptional level in breast cancer progression

Vislovukh, A.A.; Naumovets, M.G.; Kovalenko, M.I.; Groisman, R.S.; Groisman, I.S.; Negrutskii, B.S.; El'skaya, A.V. (Вiopolymers and Cell, 2013)

Eukaryotic translation elongation factor 1A exists as two 98 % homologous isoforms: eEF1A1 (A1) and eEF1A2 (A2) which are tissue and development specific. Despite high homology in an open reading frame (ORF) region, mRNAs ...
Leucine-zipper motif is responsible for self-association of translation elongation factor 1Bβ

Bondarchuk, T.V.; Shalak, V.F.; Negrutskii, B.S.; El’skaya, A.V. (Вiopolymers and Cell, 2016)

Translation elongation factor 1Bβ (eEF1Bβ) is a metazoan-specific protein catalyzing the guanine nucleotide exchange on translation elongation factor 1A (eEF1A). eEF1Bβ was reported to form oligomers. Aim. To define the ...
Mass-spectrometric and bioinformatic analysis of eEF1Bγ interactome in the cytoplasmic fraction of A549 cells

Kapustian, L.M.; Lysetsky, I.L.; Bondarchuk, T.V.; Novosylna, O.V.; Negrutskii, B.S. (Інститут молекулярної біології і генетики НАН УкраїниВiopolymers and Cell, 2018)

Aim. To study protein networks containing the translation elongation factor eEF1B gamma (eEF1Bγ) in lung carcinoma cells. Methods. The protein partners of eEF1Bγ in the cytoplasmic fraction of human lung adenocarcinoma ...
mRNAs coding for A1 and A2 isoforms of translation factor eEF1A demonstrate different half-lives while A1 and A2 proteins are similarly stable in MCF7 cells

Vislovukh, A.A.; Gralievska, N.L.; Naumovets, M.G.; Negrutskii, B.S.; El'skaya, A.V. (Вiopolymers and Cell, 2013)

Eukaryotic translation elongation factor 1A (eEF1A) exists as two 98 % homologous isoforms eEF1A1 and eEF1A2 that are tissue/development specific and differentially linked to apoptosis/cancerogenesis. A2 is overexpressed ...
Multisubunit complex eEF1H in human glial tumors: from mRNA to protein

Veremieva, M.V.; Malysheva, T.A.; Zozulya, Y.P.; Rozumenko, V.D.; Sidorik, L.L.; Kavsan, V.M.; Negrutskii, B.S.; El'skaya, A.V. (Вiopolymers and Cell, 2010)

Aim. To investigate protein level of all subunits of the eukaryotic elongation translation factor eEF1H (eEF1A, eEF1Bα, eEF1Bβ and eEF1Bγ) in glial tumors of human brain in comparison with normal brain. Methods. The eEF1H ...
Non-canonical interactions of the β subunit of the translation elongation complex eEF1B and analysis of their possible functional role

Kapustian, L.M.; Dadlez, M.; Negrutskii, B.S. (Вiopolymers and Cell, 2016)

Aim. To predict protein networks which may comprise the β subunit of the translation elongation complex eEF1B in lung carcinoma cell line. Methods. The protein partners of eEF1Bβ from cytoplasmic extract of A549 cells were ...
Protein partners of the eEF1Bβ subunit of the translation elongation complex eEF1B in the nuclear fraction of human lung carcinoma cells

Kapustian, L.M.; Dadlez, M.; Negrutskii, B.S. (Вiopolymers and Cell, 2017)

Aim. To identify novel protein partners of translation factor eEF1Bβ in nucleus of human lung carcinoma cells. Methods. Protein partners of eEF1Bβ in the nuclear fraction of A549 cells were identified by co-immunoprecipitation ...
PTI-1: novel way to oncogenicity

Vislovukh, A.A.; Shalak, V.F.; Savytskyi, O.V.; Kovalenko, N.I.; Gralievska, N.L.; Negrutskii, B.S.; El’skaya, A.V. (Вiopolymers and Cell, 2012)

Aim. The prostate tumor-inducing oncogene (PTI-1), presumably encoding a truncated form of eukaryotic translation elongation factor 1A1 (eEF1A1), was discovered as a gene overexpressed in prostate tumor samples and absent ...
Specific features of protein biosynthesis in higher eukaryotes

El'skaya, A.V.; Negrutskii, B.S.; Shalak, V.F.; Vislovukh, A.A.; Vlasenko, D.O.; Novosylna, A.V.; Lukash, T.O.; Veremieva, M.V. (Вiopolymers and Cell, 2013)

Over 40 years of studies in the field of higher eukaryotic translation are summarized in the review. Among the pioneer results obtained we should especially accentuate the following: i) discovery of the adaptation of tRNAs ...
Structural dissection of human translation elongation factor 1Bγ (eEF1Bγ): expression of full-length protein and its truncated forms

Trosiuk, T.V.; Liudkovska, V.V.; Shalak, V.F.; Negrutskii, B.S.; El’skaya, A.V. (Вiopolymers and Cell, 2014)

Aim. To gain more insights into properties of the human translation elongation factor eEF1Bγ and its interaction with partners we intended to produce the full-length protein and its truncated forms. Methods. cDNAs encoding ...
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts

Negrutskii, B.S. (Биополимеры и клетка, 1997)

Highly effective permeabilization/translation system developed recently (Negrutskii et al. (1994) Proc. Natl Acad. Sci. USA 91, 964–968) was used to study the distribution of FITC-labeled a and S submits of translation ...
Transcriptional and post-transcriptional control of eEF1A2 expression during myoblast diffrerentiation

Vislovukh, A.A.; Groisman, I.S.; El’skaya, A.V.; Negrutskii, B.S.; Polesskaya, A.N. (Вiopolymers and Cell, 2012)

During postnatal development, the switch of the expression from isoform A1 to the isoform A2 of eukaryotic translation elongation factor (eEF1A) is observed in neuronal and muscle tissues. The switch of the expression is ...

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