Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции

dc.contributor.author	Зайцева, Г.П.
dc.contributor.author	Акинина, Г.Е.
dc.contributor.author	Твердохлеб, Е.В.
dc.contributor.author	Дугарь, Ю.Н.
dc.contributor.author	Попов, В.Н.
dc.date.accessioned	2021-02-17T20:04:17Z
dc.date.available	2021-02-17T20:04:17Z
dc.date.issued	2014
dc.identifier.citation	Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции / Г.П. Зайцева, Г.Е. Акинина, Е.В. Твердохлеб, Ю.Н. Дугарь, В.Н. Попов // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2014. — Т. 14. — С. 150-153. — Бібліогр.: 11 назв. — рос.	uk_UA
dc.identifier.issn	2219-3782
dc.identifier.uri	http://dspace.nbuv.gov.ua/handle/123456789/178084
dc.description.abstract	Aims. The aim of our research was to identify Lr-genes in winter wheat Ukrainian varieties using DNA markers. Methods. DNA was isolated from a mixture of five seeds. Presence of Lr genes was studied by polymerase chain reaction (PCR). The amplification products were visualized by electrophoresis on a 2% agarose gel. For determination the numbers and sizes of the amplification products demo version of program TotalLab 120 was used. Results. Lr10 gene was detected in six varieties of winter wheat from two breeding centers – The Plant Production Institute (PPI) and The Plant Breeding and Genetics Institute (PBGI). In the studied varieties Lr19 and Lr20 genes were not identified. Lr34 gene was identified in 13 varieties of PPI, 17 varieties of PBGI and three varieties of the Mironivskiy institute of wheat (MIW). Conclusions. Using the DNA-markers varieties and lines of soft winter wheat with the Lr genes were identified. The two Lr genes, Lr10 and Lr34, are present in some studied samples. And two genes, Lr19 and Lr20, were not identified in studied Ukrainian winter wheat varieties and lines. Key words: Lr- genes, DNA markers, leaf rust, T. aestivum L.	uk_UA
dc.language.iso	ru	uk_UA
dc.publisher	Інститут молекулярної біології і генетики НАН України	uk_UA
dc.relation.ispartof	Фактори експериментальної еволюції організмів
dc.subject	Аналіз та оцінка генетичних ресурсів	uk_UA
dc.title	Определение Lr генов в сортах пшеницы мягкой озимой украинской селекции	uk_UA
dc.title.alternative	Determination of Lr genes in soft winter wheat varieties of ukrainian selection	uk_UA
dc.type	Article	uk_UA
dc.status	published earlier	uk_UA
dc.identifier.udc	633.11:575