Генетична колекція Aegilops biuncialis Vis.

Abstract

Aim. The aim of the study was to make the genetic collection of Aegilops biuncialis Vis. «Collection of Aegilops biuncialis Vis. accessions with respect to alleles at the storage protein loci Glu-U1, Glu-Mb 1, Gli-U1, Gli-Mb 1» and analyze its diversity in comparison with that in Crimean populations. Methods. We used SDS and APAG electrophoresis to identify alleles at the Glu-1 and Gli-1 loci. Results. In the Ae. biuncialis collection there are 6, 9, 13, and 12 alleles at the loci Glu-U1, Glu-Mb 1, Gli-U1 and Gli-Mb 1, respectively. In comparison with the allele diversity revealed in Crimean populations of the species, the collection represents 60% of identified alleles at Glu-U1, 50% of alleles at Glu-Mb 1, 68% of alleles at Gli-U1, and 100% of alleles at Gli-Mb 1. Conclusions. The Ae. biuncialis collection comprises from 50 to 100% of diversity of alleles at the storage protein loci revealed in Crimean populations and may be considered as the basis of the core collection from its Crimean part of the area. Keywords: Aegilops biuncialis Vis., diversity, high-molecular-weight glutenin subunits, gliadin, alleles.