Introgressive wheat lines Triticum Aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes

Abstract

Aims. Using SSR loci specifi c to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F5 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identifi ed 9 SSR loci specifi c to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5 progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a signifi cant number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from F2 . Conclusions. Microsatellite loci could be used for identifi cation of introgressions from Aegilops mutica in wheat genome (although with some limitations). Identifi ed plants with recombinant 5D/5Т chromosomes are perspective for future work. Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines, recombinant lines.