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Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro

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dc.contributor.author Kochubei, T.O.
dc.contributor.author Maksymchuk, O.V.
dc.contributor.author Piven, O.O.
dc.contributor.author Lukash, L.L.
dc.date.accessioned 2019-01-22T11:32:48Z
dc.date.available 2019-01-22T11:32:48Z
dc.date.issued 2015
dc.identifier.citation Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. uk_UA
dc.identifier.issn 1812-9269
dc.identifier.uri http://dspace.nbuv.gov.ua/handle/123456789/145466
dc.description.abstract Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax. uk_UA
dc.language.iso en uk_UA
dc.publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України uk_UA
dc.relation.ispartof Experimental Oncology
dc.subject Original contributions uk_UA
dc.title Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro uk_UA
dc.type Article uk_UA
dc.status published earlier uk_UA


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