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dc.contributor.author |
Gerashchenko, O. |
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dc.contributor.author |
Zhuravel, E. |
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dc.contributor.author |
Skachkova, O. |
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dc.contributor.author |
Khranovska, N. |
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dc.contributor.author |
Pushkarev, V. |
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dc.contributor.author |
Pogrebnoy, P. |
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dc.contributor.author |
Soldatkina, M. |
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dc.date.accessioned |
2019-01-20T10:10:19Z |
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dc.date.available |
2019-01-20T10:10:19Z |
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dc.date.issued |
2014 |
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dc.identifier.citation |
Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells / O. Gerashchenko, E. Zhuravel, O. Skachkova, N. Khranovska, V. Pushkarev, P. Pogrebnoy, M. Soldatkina // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 17-23. — Бібліогр.: 35 назв. — англ. |
uk_UA |
dc.identifier.issn |
1812-9269 |
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dc.identifier.uri |
http://dspace.nbuv.gov.ua/handle/123456789/145313 |
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dc.description.abstract |
Background and Aim: Human beta-defensin-2 (hBD-2) is an antimicrobial cationic peptide capable to control human carcinoma cell growth via cell cycle regulation. The present study was aimed on determination of hBD-2 influence on the growth patterns and malignant potential of cultured human melanoma cells. Methods: The study was performed on cultured human melanoma cells of mel Z and mel Is lines treated with recombinant hBD-2 (rec-hBD-2); cell viability, proliferation, cell cycle distribution, and anchorage-independent growth were analyzed using MTT test, direct cell counting, flow cytometry, and colony forming assay respectively. Expression and/or phosphorylation levels of proteins involved in cell cycle control were evaluated by Western blotting. Results: The treatment of mel Z and mel Is cells with rec-hBD-2 in a concentration range of 100–1000 nM resulted in a concentration-dependent suppression of cell proliferation, viability, and colony forming activity. It has been shown that rec-hBD-2 exerts its growth suppression effects via significant downregulation of B-Raf expression, activation of pRB and upregulation of p21WAF1 expression, downregulation of cyclin D1 and cyclin E resulting in cell cycle arrest at G1/S checkpoint. Conclusion: According to obtained results, hBD-2 exerts its growth suppression effect toward human melanoma cells via downregulation of B-Raf, cyclin D1 and cyclin E expression, upregulation of p21WAF1 expression and activation of pRB. Key Words: human beta-defensin-2, melanoma, proliferation, viability, cell cycle, B-Raf, anchorage-independent growth. |
uk_UA |
dc.description.sponsorship |
This work was in part supported with grant 0110U005758 of National Academy of Sciences of Ukraine “Fundamental Basis of Molecular and Cellular Biotechnologies”. |
uk_UA |
dc.language.iso |
en |
uk_UA |
dc.publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
uk_UA |
dc.relation.ispartof |
Experimental Oncology |
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dc.subject |
Original contributions |
uk_UA |
dc.title |
Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells |
uk_UA |
dc.type |
Article |
uk_UA |
dc.status |
published earlier |
uk_UA |
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