Наукова електронна бібліотека
періодичних видань НАН України

Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1

Репозиторій DSpace/Manakin

Показати простий запис статті

dc.contributor.author Magorivska, I.
dc.contributor.author Bilyy, R.
dc.contributor.author Shalay, O.
dc.contributor.author Loginsky, V.
dc.contributor.author Kit, Y.
dc.contributor.author Stoika, R.
dc.date.accessioned 2018-06-15T14:02:10Z
dc.date.available 2018-06-15T14:02:10Z
dc.date.issued 2009
dc.identifier.citation Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 / I. Magorivska, R. Bilyy, O. Shalay, V. Loginsky, Y. Kit1, R. Stoika // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 97-101. — Бібліогр.: 30 назв. — англ. uk_UA
dc.identifier.issn 1812-9269
dc.identifier.uri http://dspace.nbuv.gov.ua/handle/123456789/135699
dc.description.abstract Background: Recently we have shown that the imunoglobulins G from blood serum of some multiple sclerosis patients are capable of cleaving histone H1. Aim: To check whether histone H1-hydrolyzing abzymes could be detected not only in blood plasma of autoimmune patients, but also during cancer development, particularly during the onset of multiple myeloma. Methods: Immunoglobulines were isolated from blood serum of multiple myeloma patients (n = 11) by precipitation with 50% ammonium sulfate and tested for proteolytic activity toward linker and core calf thymus histones. Antibody preparations able to cleaved histone H1 were subjected to affinity chromatography on histone H1-Sepharose with following analysis of chromatographic fractions’ protease activity. To prove that antibody molecules are responsible for hydrolysis of histone H1, gel filtration at acidic pH with subsequent examination of protease activity of chromatographic fractions (pH-shock analysis) was used. Results: It was found that 3 of 11 antibody preparations are capable of hydrolyzing calf thymus histone H1 but not core histones. It was shown that histone H1-hydrolysing activity of 2 proteolytically active antibody preparations is associated with IgGs that possess affinity towards histone H1. pH-shock analysis proved that protease activity towards histone H1 is intrinsic property of IgG molecules. Conclusions: We demonstrated the existence of previously unknown histone H1 hydrolyzing IgG abzymes in the serum of multiple myeloma patients. Possible biological role of hisH1-hydrolyzing antibodies in patients with multiple myeloma was discussed. uk_UA
dc.language.iso en uk_UA
dc.publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України uk_UA
dc.relation.ispartof Experimental Oncology
dc.subject Original contributions uk_UA
dc.title Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 uk_UA
dc.type Article uk_UA
dc.status published earlier uk_UA


Файли у цій статті

Ця стаття з'являється у наступних колекціях

Показати простий запис статті

Пошук


Розширений пошук

Перегляд

Мій обліковий запис