Detection of biomolecules using optoelectronic biosensor based on localized surface plasmon resonance. Nanoimprint lithography approach

Abstract

In this work, we use a nanoimprint lithography fabrication technology to create uniformly-oriented and homogenous noble metal nanoparticle arrays with wellcontrolled size, shape and spacing, which can be the basis platform for the development of Localized Surface Plasmon Resonance (LSPR) sensors for biomolecular detection. Using this approach, we demonstrate proof-of-principle of an optical biosensor to quantify biomolecular interactions in a real-time mode using a UV-visible spectrophotometer. The sensor shows concentration-dependent kinetics of surface adsorption or binding of biomolecules and a capability to monitor antigen-antibody specific reactions, which is demonstrated by the reaction between bovine serum albumin (BSA) and anti-BSA immunoglobulin.