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Вiopolymers and Cell, 2015, vol. 31
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Перегляд Вiopolymers and Cell, 2015, vol. 31 за темою

Перегляд Вiopolymers and Cell, 2015, vol. 31 за темою "Molecular and Cell Biotechnologies"

Результати 1-13 з 13

Construction and analysis of the transgenic carrot and celery plants expressing the recombinant thaumatin II protein

Luchakivska, Yu.S.; Komarnytskii, I.K.; Kurchenko, I.M.; Yurieva, O.M.; Zhytkevich, N.V.; Kuchuk, M.V. (Вiopolymers and Cell, 2015)

Aim To obtain the transgenic carrot and celery plants able to express recombinant thaumatin II in order to increase plant stress tolerance. Methods. Agrobacterium-mediated transformation of the carrot and celery seedlings ...
Detection of stress resistance genes in transgenic maize by multiplex and touchdown polymerase chain reaction

Bannikova, M.A. (Вiopolymers and Cell, 2015)

Aim. To develop a methodology for detection of the genes of resistance to the stress factors in transgenic maize by multiplex (mPCR) and touchdown polymerase chain reactions. Methods. isolation of total DNA by CTAB method, ...
Development of monoclonal antibody against protein Rictor, a component of the mTORC2 complex

Malanchuk, O.M. (Вiopolymers and Cell, 2015)

Aim. Rictor is a component of the protein complex mTORC2 that is activated by growth factors and regulates cell growth, survival and migration. Here, we describe the development of the Rictor specific monoclonal antibody ...
Development of the chromatographic medium for the affinity isolation of the recombinant hIFN-β1b based on immobilized single-chain antibodies

Pokholenko, Ia.O.; Gorbatiuk, O.B.; Okunev, O.V.; Irodov, D.M.; Degtiarova, M.I.; Palivoda, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. The development of a laboratory method for the production of immunoaffinity chromatography medium for the purification of the recombinant human IFN-β1b. Methods. A gene of the chimeric protein ScFvbINF-CBD was constructed ...
Distribution of transplanted human mesenchymal stem cells from Wharton’s Jelly in the central nervous systems of the EAE rats

Kovalchuk, M.V.; Deryabina, O.G.; Pichkur, L.D.; Verbovskaya, S.A.; Shuvalova, N.S.; Pichkur, O.L.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Human Wharton’s Jelly MSCs (hWJ-MSCs) have a considerable advantage and potential in treating the central nervous system diseases and can be a new alternative treatment of Multiple Sclerosis (MS). Aim. To study the persistence ...
Effect of antibiotic ceftriaxone on elimination of ABI and GV3101 strains of Agrobacterium tumefaciens

Gorbatyuk, I.R.; Gnatyuk, I.S.; Bannikova, M.A. (Вiopolymers and Cell, 2015)

Aim. To find out, at which concentration the antibiotic ceftriaxone of β-lactam group causes the elimination of ABI and GV3101 strains of Agrobacterium tumefaciens. Methods. The disc diffusion method. Results. Antibiotic ...
Generation and characterization of polyclonal antibodies specific to N-terminal extension of p85 isoform of ribosomal protein S6 kinase 1 (p85 S6K1)

Savinska, L.O.; Klipa, O.M.; Khoruzenko, A.I.; Shkarina, K.A.; Garifulin, O.M.; Filonenko, V.V. (Вiopolymers and Cell, 2015)

Aim. Generation of polyclonal antibodies specific to the ribosomal protein S6 kinase isoform – p85S6K1 and directed to the N-terminal (1–23 aa) extension of p85S6K1. Methods. Animal immunization with synthetic (1–23 aa) ...
Influence of rat progenitor neurogenic cells supernatant on glioma 101.8 cells in vitro

Liubich, L.D.; Semenova, V.M.; Stayno, L.P. (Вiopolymers and Cell, 2015)

Aim. To evaluate the influence of the rat progenitor neurogenic cells supernatant (RPNS) on the transplantable rat malignant brain glioma cells (strain 101.8) under conditions of cultivation. Methods. Primary cultures were ...
Monitoring of transplanted human Mesenchymal Stem Cells from Wharton’s Jelly in xenogeneic systems in vivo

Kovalchuk, M.V.; Shuvalova, N.S.; Pokholenko, I.O.; Dragulyan, M.V.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation. Aim. to study the survival of transplanted human MSCs from umbilical cord Wharton’s Jelly (hWJ-MSCs) ...
Monitoring of transplanted human Mesenchymal Stem Cells from Wharton’s Jelly in xenogeneic systems in vivo

Kovalchuk, M.V.; Shuvalova, N.S.; Pokholenko, I.O.; Dragulyan, M.V.; Gulko, T.P.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation. Aim. to study the survival of transplanted human MSCs from umbilical cord Wharton’s Jelly (hWJ-MSCs) ...
Proliferation of Wharton jelly mesenchymal stem cells, derived by preserving the cells with reduced attachment rate, under various gas conditions

Shuvalova, N.S.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. To estimate the proliferation rates in cultures of the human Wharton jelly mesenchymal stem cells (WJ-MSCs), obtained by the method of preservation of spontaneously detached cells, in various gas mixtures, containing ...
Recombinant Staphylococcal protein A with cysteine residue for preparation of affinity chromatography stationary phase and immunosensor applications

Gorbatiuk, O.B.; Bakhmachuk, A.O.; Dubey, L.V.; Usenko, M.O.; Irodov, D.M.; Okunev, O.V.; Kostenko, O.M.; Rachkov, A.E.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. Engineering of recombinant Staphylococcal protein A with cysteine residue (SPA-Cys) for preparation of affinity chromatography stationary phase and formation of bioselective element of immunosensor. Methods. DNA ...
SPR investigations of the formation of intermediate layer of the immunosensor bioselective element based on the recombinant Staphylococcal protein A

Rachkov, A.E.; Bakhmachuk, A.O.; Gorbatiuk, O.B.; Matsishin, M.J.; Khristosenko, R.V.; Ushenin, Iu.V.; Soldatkin, A.P. (Вiopolymers and Cell, 2015)

Aim. To investigate the formation of an intermediate layer of the immunosensor bioselective element based on the recombinant protein A from Staphylococcus aureus with cysteine residue (SPA-Cys) and its interactions with ...

Результати 1-13 з 13

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Перегляд Вiopolymers and Cell, 2015, vol. 31 за темою "Molecular and Cell Biotechnologies"

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Перегляд Вiopolymers and Cell, 2015, vol. 31 за темою "Molecular and Cell Biotechnologies"

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