Перегляд за автором "Tykhonkova, I.O."

Сортувати за: Порядок: Результатів:

  • Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1 
    Savinska, L.O.; Klipa, O.M.; Demchuk, N.O.; Ovcharenko, G.V.; Malanchuk, O.M.; Tykhonkova, I.O.; Palchevskyy, S.S.; Filonenko, V.V. (Вiopolymers and Cell, 2012)
    The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1, which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. ...
  • Generation of monoclonal antibody against protein phosphatase 5 
    Malanchuk, O.M.; Ovcharenko, G.V.; Tykhonkova, I.O.; Khoma, O.S.; Chorna, U.V.; Rybak, M.Yu.; Palchevskyy, S.S. (Вiopolymers and Cell, 2013)
    Aim. Serine/threonine protein phosphatase 5 (PP5) is a unique member of serine/threonine phosphatase family, comprises a regulatory tetratricopeptide repeat (TPR) domain and regulates many cell signaling pathways. Here, ...
  • Identification of tumor-associated antigens in human thyroid papillar carcinoma 
    Rodnin, N.V.; Tykhonkova, I.O.; Kyyamova, R.G.; Garifulin, O.M.; Gout, I.T.; Filonenko, V.V. (Біополімери і клітина, 2003)
    In this study two cDNA expressing libraries generated from thyroid papillar carcinomas were screened using SEREX approach. Thirty positive cDNA clones representing seventeen different genes were identified from both ...
  • In vitro model for study the interaction between tumor and stromal cells 
    Shkarina, K.A.; Cherednyk, O.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2013)
    Aim. To develop a model to study the interaction between tumor and stromal cells in three-dimensional culture. Methods. Cultivation of HeLa cell lines and human dermal fibroblasts in monolayer and three-dimensional culture, ...
  • Optimization of cell motility evaluation in scratch assay 
    Gotsulyak, N.Ya.; Kosach, V.R.; Cherednyk, O.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2014)
    A scratch test is one of the most popular methods of classical cell migration assay in a monolayer culture. At the same time, the scratch assay has some disadvantages that can be easily corrected. Aim. Optimization the ...
  • Optimization of in vitro model for analysis of tumor cell migration dynamics 
    Kravchenko, A.O.; Kosach, V.R.; Shkarina, K.A.; Zaiets, I.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2018)
    Migration ability is an important feature of tumor cells. There are several approaches to analyze the dynamics of cancer cell migration in vitro. One of the most perspective and closer to the in vivo conditions is the model ...
  • Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase 
    Kosach, V.R.; Tykhonkova, I.O.; Cherednyk, O.V.; Filonenko, V.V.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2016)
    Aim. To study the subcellular localization of phospho-mTOR (Ser2481) in cultured cells of human cancer cell lines. Methods. Immunofluorescent analysis of phospho-mTOR (Ser2481) subcellular localization in cultured MCF-7 ...