Перегляд за темою "Methods"

Сортувати за: Порядок: Результатів:

  • Comparison of proliferative activity of Wharton jelly mesenchymal stem cells in cultures under various gas conditions 
    Shuvalova, N.S.; Kordium, V.A. (Вiopolymers and Cell, 2015)
    Aim. To optimize the cultivation of Wharton jelly-derived mesenchyma stem cells (WJ-MSCs) using physiological oxygen concentrations, and to compare the effect of “hypoxic” gas mixtures, based on nitrogen and argon, on their ...
  • Development of MLPA approach for SNP detection in MTHFR, F5 and F2 genes 
    Kravchenko, S.A.; Chernushyn, S.Yu.; Kucherenko, A.M.; Soloviov, O.O.; Livshits, L.A. (Вiopolymers and Cell, 2015)
    Single nucleotide polymorphism (SNP) is an important and valuable form of DNA variation among individuals. Various methods for SNP genotyping have been developed, but a few are suitable for the flexible and low-cost ...
  • In vitro model for study the interaction between tumor and stromal cells 
    Shkarina, K.A.; Cherednyk, O.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2013)
    Aim. To develop a model to study the interaction between tumor and stromal cells in three-dimensional culture. Methods. Cultivation of HeLa cell lines and human dermal fibroblasts in monolayer and three-dimensional culture, ...
  • Maintenance of mesenchymal stem cells culture due to the cells with reduced attachment rate 
    Shuvalova, N.S.; Maslova, O.A.; Sukhorada, O.M.; Deryabina, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2013)
    Aim. The classic detachment techniques lead to changes in cells properties. We offer a simple method of cultivating the population of cells that avoided an influence on the surface structures. Methods. Mesenchymal stem ...
  • Optimization of cell motility evaluation in scratch assay 
    Gotsulyak, N.Ya.; Kosach, V.R.; Cherednyk, O.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2014)
    A scratch test is one of the most popular methods of classical cell migration assay in a monolayer culture. At the same time, the scratch assay has some disadvantages that can be easily corrected. Aim. Optimization the ...
  • Optimization of in vitro model for analysis of tumor cell migration dynamics 
    Kravchenko, A.O.; Kosach, V.R.; Shkarina, K.A.; Zaiets, I.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2018)
    Migration ability is an important feature of tumor cells. There are several approaches to analyze the dynamics of cancer cell migration in vitro. One of the most perspective and closer to the in vivo conditions is the model ...
  • Optimization of tumor cell culture conditions in soft agar for subsequent immunohistochemical analysis 
    Khoruzhenko, A.I. (Вiopolymers and Cell, 2012)
    Мета. Оптимізувати умови культивування злоякісних клітин у напіврідкому агарі для подальшого імуногістохімічного аналізу отриманих тривимірних колоній клітин. Методи. Культивування клітин лінії MCF-7 карциноми молочної ...
  • Topokaryotyping – a proposal for a novel approach to study nuclear organization 
    Jurisic, A.; Robin, C.; Ogryzko, V. (2015)
    Gene positioning in the nucleus plays an important role in gene activity and genome stability, both in norm and pathology. We propose a new principle for the analysis of the large scale chromatin organization at the ...