Прихована хромосомна нестабільність, виявлена при тестуючій мутагенній дії блеоміцину in vitro в лімфоцитах периферичної крові контрольних донорів

Abstract

A model system for the investigation of a hidden chromosome instability in somatic human cells by means of bleomycin testing mutagenic exposure in vitro is elaborated. Optimal terms of the treatment by bleomycin of human peripheral blood lymphocytes culture (late post-synthetic G2 phase of mitotic cycle) as well as optimal concentrations of bleomycin (0.05 аnd 5.0 μg/ml) for the evaluation of human chromosomes sensitivity to mutagenic exposure in vitro are suggested. It is shown that the main criterion of chromosomes sensitivity to bleomycin exposure must be the total frequency of chromosome aberrations (but not aberrant metaphases). With the help of modifying “G2-bleomycin sensitivity assay”, the investigation of hidden chromosomes instability in 9 healthy donors is fulfilled. Three hypersensitive persons had been identified that can be considered as the genetically caused phenomenon.