Experimental Oncology, 2014, № 3http://dspace.nbuv.gov.ua:80/handle/123456789/1331612024-03-28T11:57:35Z2024-03-28T11:57:35ZHuman papilloma virus and nasopharyngeal carcinoma: pathology, prognosis, recurrence and mortality of the diseaseAtighechi, S.Mirvakili, S.A.Sheikhha, M.H.Baradaranfar, M.H.Dadgarnia, M.H.Behniafard, N.Ahmadpour Baghdadabad, M.R.http://dspace.nbuv.gov.ua:80/handle/123456789/1453702019-01-21T23:23:22Z2014-01-01T00:00:00ZHuman papilloma virus and nasopharyngeal carcinoma: pathology, prognosis, recurrence and mortality of the disease
Atighechi, S.; Mirvakili, S.A.; Sheikhha, M.H.; Baradaranfar, M.H.; Dadgarnia, M.H.; Behniafard, N.; Ahmadpour Baghdadabad, M.R.
Background: One of the malignant tumors among head and neck cancers is nasopharyngeal carcinoma. Many studies consider human papilloma virus (HPV) as a cause for nasopharyngeal carcinoma. Methods: 41 paraffin-wax-embedded block samples were examined to detect HPV DNA and its subtype’s presence by polymerase chain reaction. The recurrence, prognosis and survival were evaluated for an average of 48 months. Results: HPV DNA was positive in 9 patients (22%). The overall recurrence rate was 75% in HPV negative patients and 11% in HPV positive ones. The mortality rate in HPV negative and positive patients was 37.5% and 0%, respectively. Conclusion: HPV type 18 and 16 were the most common subtypes. Also, it can be implied that patients which are HPV positive had better prognosis and also less recurrence. Key Words: human papilloma virus, nasopharyngeal cancer, polymerase chain reaction, prognosis, recurrence.
2014-01-01T00:00:00ZIn vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixtureRoomi, M.W.Bhanap, B.Roomi, N.W.Niedzwiecki, A.Rath, M.http://dspace.nbuv.gov.ua:80/handle/123456789/1453692019-01-21T23:23:20Z2014-01-01T00:00:00ZIn vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
Roomi, M.W.; Bhanap, B.; Roomi, N.W.; Niedzwiecki, A.; Rath, M.
Aim: Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metastasis. Biological agents that prevent extracellular matrix digestion by the MMPs have been shown to be promising therapeutic approaches to cancer. In this study, we investigated effects of a nutrient mixture (NM) containing, ascorbic acid, lysine, proline and green tea extract, on human FANCA and FANCC lymphoblasts for viability, MMP secretion and invasion. Methods: Human FANCA lymphoblasts GM13022 and HCS536 were challenged with NM at concentration range within 10–1000 µg/ml. Cell toxicity was assessed by Trypan blue dye exclusion test. Invasion was evaluated through Matrigel and gelatinase zymography for MMP activity. Results: NM was toxic in dose dependent mode to HCS536 cells but not to GM13022 cells. GM13022 cells but not HCS536 cells exhibited MMP-9 secretion, which was inhibited by NM. Matrigel invasion was inhibited in HCS536 cells at 100 and 500 µg/ml by 27% and 93%, respectively. In GM13022 cells, the NM showed completely blocked Matrigel invasion at 500 µg/ml. Conclusion: NM inhibited MMP secretion and Matrigel invasion in FANCA and inhibited invasion and induced toxicity in FANCC lymphoblasts. These results suggest that the NM may have therapeutic potential in Fanconi anemia associated neoplasia. Key Words: Fanconi anemia, nutrient mixture, MMP, Matrigel invasion.
2014-01-01T00:00:00ZRisk assessment of cancer of the female reproductive systemGlushchenko, N.M.Nesina, I.P.Lurchenko, N.P.Proskurnya, L.A.Buchynska, L.G.http://dspace.nbuv.gov.ua:80/handle/123456789/1453682019-01-21T23:23:19Z2014-01-01T00:00:00ZRisk assessment of cancer of the female reproductive system
Glushchenko, N.M.; Nesina, I.P.; Lurchenko, N.P.; Proskurnya, L.A.; Buchynska, L.G.
Aim: To create an information resource concerning multifactorial oncological diseases of the female reproductive system. Materials and Methods: A comprehensive search of the literature in the PubMed and Ukrainian scientific sources published from 1995 to 2014 and the results of researches performed in R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine. Development environment of information resource “Multifactorial oncological disease” was Borland Delphi. Results: The information content of web page concerning cancers of the female reproductive system was posted in the information resource “Multifactorial oncological disease”. The assessment algorithm of genetic contribution to cancers of the female reproductive system and recurrent risk of cancer development in families have been described. These algorithms can be used in assessment of contribution of genetic and environmental factors in the development of malignant tumors. Key Words: cancers of the female reproductive system, information resource, genetic contribution, recurrent risk.
2014-01-01T00:00:00ZThe study of chromosomal instability in patients with endometrial cancerNesina, I.P.Lurchenko, N.P.Nespryadko, S.V.Buchynska, L.G.http://dspace.nbuv.gov.ua:80/handle/123456789/1453672019-01-21T23:23:16Z2014-01-01T00:00:00ZThe study of chromosomal instability in patients with endometrial cancer
Nesina, I.P.; Lurchenko, N.P.; Nespryadko, S.V.; Buchynska, L.G.
Aim: Study is devoted to evaluation of sensitivity of peripheral blood T-lymphocytes (PBL) of patients with endometrial cancer (EC) to genotoxic effect of bleomycin and detection of patients with hidden chromosomal instability. Methods: PBL of 24 EC patients (mean age 58.9 ± 2.9) and 10 healthy women-volunteers (mean age 55.7 ± 2.3) were subjected to cytogenetic analysis. Results: Mean spontaneous level of chromosomal aberrations (CA) per 100 analyzed lymphocytes (CA/100) of healthy women has equaled 2.7 ± 0.6, i.e. has not exceeded maximal values of healthy population and was significantly lower (p < 0.05), than in PBL of EC patients (6.9 ± 0.6). After incubation of PBL with bleomycin, number of CA/100 significantly was increased both in control (11.5 ± 1.3) and in EC patients (21.9 ± 1.0). Spontaneous chromosomal instability has been observed in 41.7%, increased sensitivity to bleomycin — in 54.2% and hidden chromosomal instability in 37.5% of patients with EC. It has been shown that level of specific damage of genome in EC patients has constituted 2·10–5, and after exposure with bleomycin, it was increased 4.5 times (9·10–5) that was significantly higher (p < 0.05) compared to control (8·10–6 and 1.0·10–5, respectively). Conclusion: These results have demonstrated that PBL of most patients with EC are characterized by apparent genome alterations, which are manifested by increased number of spontaneous and induced chromosomal damages, hypersensitivity to mutagens and hidden chromosomal instability. Key Words: endometrial cancer, peripheral blood lymphocytes, chromosomal aberrations, hidden chromosomal instability.
2014-01-01T00:00:00Z